Monday - 9:30 - 17:30




-Participant and instructor introductions, housekeeping and course overview


-Hands-on introduction to UNIX and command line computing


-The history and ecology of eDNA. Overview of applications across disciplines


-Hands-on introduction to R





Tuesday - 9:30 - 17:30




-Theoretical introduction to single species detection (qPCR, ddPCR etc.)


-Hands-on design of qPCR assays





Wednesday  -  9:30 - 17:30




-Introduction to metabarcoding


-Hands-on analysis of metabarcoding data using OTU clustering


-Hands-on analysis of metabarcoding data using DADA2 denoiser





Thursday -  9:30 - 17:30




-Quality control and dataset refinement of metabarcoding data


-Assembling and curating reference libraries


-Taxonomic assignment of unknown DNA sequences


-Using high-throughput sequencing to answer ecological questions





Friday -  9:30 - 17:30



-Project day