Monday 28th – Classes from 09:30 to 17:30


 Lecture 1 – Genome editing introduction

•      General introduction
•      History of genome editing
•      Genome editing terminology
•      Survey of commonly-used genome editing nucleases

 Lab 1 –  Genome editing applications

•      Small-group discussion of ethical considerations of genome editing
•      Recent literature search

 Lecture 2 – sgRNA guide design and analysis

•      sgRNA guide design
•      Editing activity prediction
•      Assays for determining editing activity

 Lab 2 –  Performing sgRNA guide design

•      Genome feature exploration using UCSC Genome Browser
•      sgRNA guide design using Cas-designer and CRISPOR

 Tuesday 29th – Classes from 09:30 to 17:30


 Lecture 3 – Off-target analysis

•      Importance of detection of CRISPR off-targets
•      Computational prediction of off-targets
•      Biological assays for finding off-targets
•      Considerations for off-target identification

 Lab 3 –  Performing off-target analysis

•      Predicting off-targets using CasOffinder
•      Interpreting Digenome-seq data using IGV

 Lecture 4 – Confirmation of on- and off-targets

•      Computational analysis of Sanger assays
•      Computational analysis of NGS assays

 Lab 4 – Performing confirmation of on- and off-targets

•      Analyzing Sanger sequencing using ICE/TIDE
•      Analyzing NGS sequencing using CRISPResso2

 Wednesday 30th – Classes from 09:30 to 17:30

 
 Lecture 5 – Pooled screens

•      CRISPR pooled experimental strategies
•      Pooled screen design
•      Pooling screen analysis
•      Tiling screen design
•      Tiling screen analysis

 Lab 5 –  Analyzing pooled screens

•      Analysis of genome-wide screens using MAGeCK
•      Design of tiling screens using CRISPOR
•      Analyzing tiling modalities using CRISPR-SURF

 Lecture 6 – Open Discussion

•      Review, Questions and Answers
•      Analysis of attendee data